Prothrombin Time (PT)

Sample for Prothrombin Time (PT)

• The blood sample is taken with ESR (sodium citrate) solution.
  • The blood sample is 1.8 ml, and the ESR solution is 0.2 ml.

Precautions for Prothrombin Time (PT)

1. Alcohol intake can increase PT time.
2. A high-fat diet may decrease PT time.
3. Some drugs decrease PT, like anabolic steroids, digitalis, Benadryl, griseofulvin, oral contraceptives, vitamin K, and chloral hydrate.
4. Some drugs can increase PT, like aminosalicylic acid, barbiturates, beta-lactam antibiotics, chloramphenicol, clofibrate, heparin, methyldopa, neomycin, quinidine, quinine, sulphonamides, and salicylates.

Indications for Prothrombin Time (PT)

1. This is one of the screening tests for coagulation.
2. This test evaluates the extrinsic pathway defect.
3. This test also finds a common pathway defect.
4. This test is done For the screening of prothrombin deficiency.
5. PT is also used to evaluate dysfibrinogenemia.
6. PT is done to monitor the heparin and coumarin effect.
7. In the case of bruises and abnormal bleeding.
8. In the case of Vit. K deficiency.
9. This is advised as a liver function test.
   1. This is used to assess synthetic liver function.
10. To monitor the Warfarin therapy.

Pathophysiology of Prothrombin Time (PT)

1. Prothrombin is a protein produced by the liver. Its molecular weight is 71,600 Daltons.
   1. Prothrombin helps in the clotting mechanism.
   2. Prothrombin production depends upon the adequate intake amount of Vit. K and its absorption.
   3. It is the most abundant and has a long half-life of the vit. K dependant clotting proteins circulate as a zymogen to serine protease.
2. On clotting, prothrombin is converted into thrombin.
   1. Prothrombin   Thrombin.

3. Factor II, VII, IX, and X depend upon the Vit. K for biosynthesis.

Prothrombin facts:

1. The abnormality in prolonged Prothrombin test (PT):
   1. Factors I, II, VII, and X deficiency.
   2. Warfarin, liver diseases, and it. K deficiency.
2. Prothrombin Time (PT) measures :
   1. Extrinsic pathway (Activated VIIa)
   2. Tissue factor.
   3. Common pathways ( factor X, V, II, and fibrinogen ).

Principle of prothrombin time (PT):

Clotting Mechanism

1. The first stage is an aggregation of platelets to plug the damaged blood vessels.
2. The second stage is by activation of clotting factors.
   1. The first phase of the activation of the intrinsic pathway.
   2. At the same time, the extrinsic pathway is activated.
   3. By common pathway, factor X is activated by the proteases formed by the intrinsic and extrinsic pathways.
   4. The fourth phase is the conversion of prothrombin to thrombin in the presence of Factor X, Factor V, phospholipids, and calcium.
   5. Thrombin converts fibrinogen to fibrin.

3. Fibrin is converted into a stable gel.
4. XIII cross-links the fibrin and forms the clot.

NORMAL Prothrombin Time (PT)

• PT = 11 to 13 seconds (normally PT is 85% to 100%)
  • (This may vary from lab to lab).
  • This should be compared with the control, which will be around 11 to 14 seconds.
  • Anticoagulant therapy response =  1.5 to 2 times the control value.
  • Critical value = >20 seconds
• INR is an international normalized ratio. = 0.8 to 1.1

Increased Prothrombin Time (PT) is seen in the following:

1. Deficiency of factor II (prothrombin), V, VII, and X.
2. Liver diseases, like cirrhosis and hepatic failure.
   1. Factor I, II, V, VII, IX, and X are produced in the liver.
   2. This is abnormal when there is a severe disease of the liver.
3. Biliary obstruction. There is a lack of bile which is needed for fat-soluble vitamins like Vit. A, D, E, and K for their absorption.
   1. Synthesis of factors II, VII, and X depends upon the vitamin K.
   2. Suppose the patients respond to Vit. K therapy in 1 to 3 days means no liver cell damage; rather, there is a biliary obstruction.
   3. If there is no response to Vit. k therapy, the patient has severe liver cell injury.
4. Sprue, celiac disease, and chronic diarrhea.
5. Vit. K deficiency, even in the newborn of the mother with Vit. K deficiency.
6. Hemorrhagic disease of the newborn.
7. Anticoagulant therapy (Warfarin and Coumadin).
8. D I C.
9. Factor 1 deficiency (Hypofibrinogenemia).
10. Dysfibrinogenemia.
11. Circulating anticoagulants are seen in lupus disease.
12. Premature newborn.
13. Salicylates intoxication.
14. Massive blood transfusion.

Summary of Prothrombin Time (PT) and  Partial thromboplastin time (PTT):

1. Abnormal PTT alone
   1. Bleeding due to deficiency of factors V11, IX, and XI.
2. Abnormal PT only
   1. Factor VII deficiency.
3. Abnormal PT and PTT both
   1. Anticoagulant therapy.
   2. D I C.
   3. Vit. K deficiency.
   4. Liver diseases.
   5. Massive blood transfusion.
   6. Rarely due to Dysfibrinogenemia and deficiency of factors X, V, and II.

• Please, for more information, see PTT and APTT.

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