Mycobacterium Tuberculosis:- Part 7 – Tuberculosis Test by ICT (Immunochromatography technique)

Sample

• This is done in the serum of the patient.

Indication

1. For the diagnosis of Tuberculosis.
2. This is a serological method.

The principle of the method

1. The ICT Tuberculosis test is an in vitro immunodiagnostic tool for the detection of antibodies to Mycobacterium tuberculosis in serum, plasma, and other body fluids.
2. The test utilizes 5 highly purified antigens which are secreted by Mycobacterium tuberculosis during an active infection.
3. Procedure:
   1. When 30 µl of serum or plasma is added to the blue pad strip.
   2. Now the antibody will bind to these antigen lines.
      1. If human IgG against TB is present. Upon closure of the test card, anti-human IgG labeled with ‘colloidal gold’ particles will diffuse binds to the bound human IgG antibodies producing one or more PINK lines.
      2. In the negative case, there will be no pink line.
      3. Also, run the control.

Result

• Negative = In nontuberculous patients, there will be no Pink line.

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