Sample

1. Malarial parasite ( MP ) may be diagnosed from a blood smear of a patient with a fever.
   1. The best time to make smear is during shivering.
   2. Make thick and thin blood smears.
2. Serum needed for a Serological method and for PCR.

P. Malariae

1. This was recognized by the Greeks because of its fever presentation.
2. This is a cosmopolitan parasite but not seen everywhere.
3. 2-3% in Africa.
   1. sporadic in Asia and S America.
      1. It is common in regions of tropical Africa.
   2. Also seen in India, Sri Lanka, Malaya, Java, New Guinea, and Europe.
4. P. malariae represents only a small percentage of infections.
5. Vector: These are seen in wild animals. Chimpanzees are also infected.

Exoerythrocytic cycle

1. It is completed in the liver in 13 to 16 days.

Erythrocytic cycle

1. Ring form typically occupies 1/6 of the infected RBC.
   1. It is smaller than P. vivax.
   2. There heavy chromatin dot.
   3. Pigments forms early.

2. Trophozoites are typically non-ameboid, with solid cytoplasm.
   1. The cytoplasm shows dark brown pigments that may mask the chromatin.
3. Schizonts typically contain 6 to 12 merozoites.
   1. These are arranged in rosettes or in irregular clusters.
   2. Central brown-green pigment may often be visible.
   3. Parasite completely fills the RBC.
4. Microgametes are similar to P. vivax with exception of one notable darker pigment and it is coarse.
5. Macrogametocytes has also darker pigments and more coarse.

6. No Schuffner’s dots are seen.

Clinical presentations

1. There is an incubation period of 18 to 40 days.
2. There is flue like symptoms.
3. The cyclical paroxysms occur every 72 hours.
4. There may be spontaneous recovery.
5. No relapse is reported.

 Diagnosis

1. History of the patient in suspected areas.
2. Blood smear:
   1. Make a blood smear when the patient has a fever. Thin and Thick smears are made.
   2. The thick smear is more helpful to find M.Parasites.
      1. The thin smear is good to identify the type of malarial parasite.
   3. Collect blood 6 to 8 hourly till 48 hours to declare negative for malaria.
   4. Giemsa stain is the best choice.
3. Serologic methods are based on immunochromatic techniques. Tests most often use a dipstick or cassette format and provide results in 2-15 minutes.
4. Polymerase chain reaction (PCR): Parasite nucleic acids are detected using the PCR technique. This is more sensitive than smear m

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