Solutions:- Part 5 – Hematoxylin solution Preparation
Reagents needed are :
-
- Hematoxylin powder (Harris hematoxylin is preferred).
- Good hematoxylin powder is brown powder.
- Potash alum (Pattkari known in Urdu).
- Mercuric oxide.
- Ethanol.
- Distal water.
- Hematoxylin powder (Harris hematoxylin is preferred).
Steps to make:
- Weigh hematoxylin 4 G for 900 mL solution.
- Weigh Mercuric oxide 2 G for 900 mL solution.
- Weigh potash 100 G for 900 mL solution.
- Measure 60 mL ethanol.
- Distal water 800 mL.
- Boil 800 mL water and add Potash alum till it is dissolved.
- Mix 4 grams hematoxylin in 60 mL ethanol. Shake well to dissolve it.
- When potash is dissolved now add the solution of hematoxylin + ethanol solution.
- Be Careful to add ethanol dissolved hematoxylin because there are chances for fire or bursting of the Potash alum solution.
- So remove the potash alum from the heater and now add hematoxylin slowly by trickling alongside the beaker.
- After adding hematoxylin solution now on low heat and add mercuric oxide.
- Keep on heating on slow heat till mercuric oxide is dissolved and now the color of the solution will give pinkish hue.
- Adjust the timing of hematoxylin color for staining of the slides which may be from 1 minute to 3 minutes with this solution.
- Time may vary from solution to solution and lab to lab.
Quit helpful thank you so much
Thanks.
Yes it was helpful…Thank you!
Thanks.
Good evening sir.
I have some problem with hematoxilin because I feel the tissue is not taking colour even I keep them for 10 minutes in hematoxilin. Same procedure for preparation I followed u mentioned above. Please guide me how can I solve this problem.
Please check your hematoxylin powder, which may not be of good quality. Also follow all steps which I have told in the preparation of the solution.