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Common Lab Errors, and Effect of various Anticoagulants on Tests

Common Lab Errors, and Effect of various Anticoagulants on Tests
September 24, 2022Chemical pathologyLab Tests
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Common Lab Errors

Effect of various Anticoagulants on Tests

  • Handling the specimens is critical to decreasing the error in the lab results. There are the following areas where the mistake can lead to erroneous laboratory results.

Indications for ordering laboratory tests:

  1. To confirm a clinical impression or to make a diagnosis.
  2. To rule out diagnosis or disease.
  3. To follow the prognosis of the disease.
  4. To help in the therapy of various diseases.
  5. For the screening of the disease in the population.

The chemical analysis involves many steps and components. these steps involved are:

  1. Measurement of the analytes.
  2. Sample pretreatment.
  3. Measurement of the reagent.
  4. Reagent and sample test mixing.
  5. Incubation of the tests.
  6. Wait for the required time.
  7. Give time for the reaction.
  8. Calculation of the test results.
  9. The final end is reporting and release of the results.

Accuracy and reproducibility of the analysis involve:

  1. Various steps in the procedure.
  2. Check and maintain the instruments.
  3. Maintain quality assurance with internal/external quality control.
  4. It may help to run quality control with the patient sample.

False lab results may be due to:

  1. Clerical mistakes.
  2. Improper performance of the tests.
  3. Instruments failure.
  4. Quality of the poor reagents.

Various factors leading to lab errors in the patient’s tests are:

  1. Preanalytical errors:
    1. Proper patient identification.
    2. Proper collection of the sample.
    3. Handling of the sample.
    4. Strict rejection policies.
    5. Exposure to heat, light, evaporation, and exposure to the atmosphere changes the result values.
    6. Light exposure to bilirubin causes photodegradation, and heat will change the enzyme activity.
    7. Exposure of plasma to a high temperature lowers the potassium value.
  2. Specimen collection:
    1. Wrong blood collection techniques like too much pressure on the blood collection area.
    2. Blood is put into the wrong test tubes for serum in the test tube containing anticoagulant.
    3. The blood sample was wrongly labeled.
  3. Patients preparation:
    1. Blood was collected at the wrong time.
    2. If a blood sample needed a fasting sample and collected at a random time.
    3. There is a diurnal variation in the various blood samples. You may collect a sample at the peak level.
  1. Specimen handling:
    1. If the blood sample is delayed in the transfer to the lab.
    2. A blood sample is stored at the wrong temperature.
    3. Did not properly process the blood sample.
  2. Chemical analysis:
    1. If technicians are not following quality control rules.
    2. If technicians are not following the protocol of the test procedure.
    3. There will be a difference between manual and automation.
  3. Reporting of the result:
    1. Mistakes in the calculation of the result.
    2. The difference in reporting the result on the phone and on paper.
possible sources of Lab errors

possible sources of Lab errors

The most common lab errors in the collection of the samples and reporting are:

  1. Wrong labeling of the sample.
  2. The technique of the blood sample:
    1. This is very important to follow an excellent technique to collect good quality blood.
    2. If you apply a tourniquet for a longer time, that will lead to acidosis and hemoconcentration.
  3. The wrong sample of the different patients or not the proper identification of the patient.
  4. The wrong ratio of the blood and the anticoagulant.
  5. Not proper mixing of the blood may lead to the micro-clot formation.
  6. Keeping the sample at an extreme (hot) temperature.
  7. There may be hemolysis, which is not a good sample for electrolytes, and so many other tests.
    1. Try to collect an adequate blood sample and avoid microclots.
  8. If there is a lipemic serum that will interfere with biochemical reactions.
  9. Wrong timing of the sample for some special tests like hormones etc.
    1. If a blood sample is taken after the food ingestion, then:
      1. The glucose level will be high.
      2. Will raise triglyceride.
      3. Will raise lipid fractions.
  10. Improper centrifugation of the blood will not give a good clear serum.
  11. If the sample is not stored at the appropriate temperature.
  12. Some tests are influenced by the light in the blood, like bilirubin.
  13. The delayed performance of the tests.
  14. Human errors can also account for laboratory variation. This depends upon the performance of the testing.
    1. This can be minimized by automation.
  15. There will be mistakes if:
    1. Selecting a specimen to analyze from the wrong patient.
    2. There are transcriptional errors.
    3. There are any mistakes along the whole chain of collecting, processing, analyzing, and reporting.
Common lab errors

Common lab errors

The sample should be rejected when it is found:

  1. Hemolysis: The sample showing hemolysis should be rejected.
    1. RBCs release analytes and enzymes into the blood/serum.
    2. It increases potassium, phosphate, and prostatic acid phosphatase.
    3. Cholesterol in case of severe hemolysis.
    4. SGOT, SGPT, creatine kinase, iron, and magnesium may also be affected.
  2. Lipemia: The serum showing lipemia should be rejected. This may cause:
    1. Decreased in sodium, potassium, and anion gap.
    2. Turbidity of the lipemic serum interferes with photometry.
    3. Fat replaces the water, which will change the electrolytes’ distribution.
    4. Triglycerides >1500 mg/dL make serum turbid and milky.
    5. Serum iron may also be decreased.
Serum appearances

Serum appearances

  1. When there are micro-clots in the blood sample.
  2. When the sample is too small to perform the test.
  3. When too much force is applied to draw the blood.
  4. If the specimen is contaminated.
  5. If the container is leaking.
  6. Faulty transportation is like very hot or cold weather.
  7. Unlabelled sample.

Effects of various anticoagulants on different tests:

Anticoagulant Inhibit  the action  Decrease the value Increase the value Stimulator effect Distort the morphology
Oxalate Alkaline phosphatase Calcium Sodium —- Blood cells
Acid phosphatase —- Potassium —- —
LDH —- —- —- —
Amylase — —- — —
Citrate
 SGPT Amylase Sodium Acid phosphatase —
SGOT Calcium Potassium —- —
Alkaline phosphatase —- —- —- —
EDTA
 Creatine Kinase Calcium PT —- —
Alkaline phosphatase Iron PTT —- —
platelet aggregation Sodium —- —
— Potassium —- —

Heparin

 

 — T 3 —- —
— Thyroxine —- —
— PT and PTT —- —
— Lithium —- —
— Sodium —- —
Fluoride Acid phosphatase — Cell morphology
Alkaline phosphatase —
SGPT —
SGOT —
Creatine kinase —
Amylase

Precautions before performing the test:

  1. This is best to perform all the tests within the first 45 minutes to one hour after collection.
  2. If delayed, then correctly store the sample at the appropriate temperature.
  3. The serum is needed for electrophoresis.
  4. For gases and ammonia, whole blood is needed.
  5. The serum is the best option for clinical chemistry, but plasma may be used.
  6. For plasma preparation, centrifuge the blood within one hour of sample collection for 10 minutes at 850 to 1000 x gravity.
  7. Cap the bottle to avoid evaporation.
  8. Keep the sample in the colored container for bilirubin estimation.

Laboratory work should know:

  1. Misidentification of the sample.
  2. Reject hemolysed sample.
  3. Avoid exposure of the sample to light and heat.
  4. Avoid evaporation of the sample.
Possible References Used
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