α-Hydroxybutyrate Dehydrogenase (HBDH)

Sample

1. This is done on the serum of the patient.
2. Separate serum as soon as possible.
3. Avoid hemolysis and use a clear serum.
4. Plasma Is not recommended.

Purpose of the test (Indications)

• For the diagnosis of myocardial infarction.

Pathophysiology

1. The HBDH is alternate to determine the  LDH isoenzymes.
   1. Roasalki and Wilkinson advocated the ratio of LDH/HBDH.
   2. In healthy individual serum, the ration of LDH/HBDH varies from1.2 to 1.6.
      1. in liver parenchymal diseases is 1.6 to 2.5.
      2. While in heart disease (Myocardial infarction)  when LDH-1 and LDH-2 activity are increased and at the same time HBDH is also increased, then the LDH/HBDH ratio is decreased to 0.8 to 1.2.
      3. LDH/HBDH ratio is more popular in the UK than in the USA.
2. The level of HBDH is usually detected within 12 hours of the infarction.
3. The peak level is seen from 48 to 72 hours.

Normal

• At 25 C = 68  to 135 U/L.
• At 30 C = 82 to 163 U/L.
• At 37 C = 96 to190 U/L.

The increased level is seen in:

1. Myocardial infarction.
2. Muscular dystrophy.
3. Megaloblastic anemia.
4. Disseminated metastasis.