α-Hydroxybutyrate Dehydrogenase (HBDH)
- This is done on the serum of the patient.
- Separate serum as soon as possible.
- Avoid hemolysis and use a clear serum.
- Plasma Is not recommended.
Purpose of the test (Indications)
- For the diagnosis of myocardial infarction.
- The HBDH is alternate to determine the LDH isoenzymes.
- Roasalki and Wilkinson advocated the ratio of LDH/HBDH.
- In healthy individual serum, the ration of LDH/HBDH varies from1.2 to 1.6.
- in liver parenchymal diseases is 1.6 to 2.5.
- While in heart disease (Myocardial infarction) when LDH-1 and LDH-2 activity are increased and at the same time HBDH is also increased, then the LDH/HBDH ratio is decreased to 0.8 to 1.2.
- LDH/HBDH ratio is more popular in the UK than in the USA.
- The level of HBDH is usually detected within 12 hours of the infarction.
- The peak level is seen from 48 to 72 hours.
- At 25 C = 68 to 135 U/L.
- At 30 C = 82 to 163 U/L.
- At 37 C = 96 to190 U/L.
The increased level is seen in:
- Myocardial infarction.
- Muscular dystrophy.
- Megaloblastic anemia.
- Disseminated metastasis.