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Urine 24 hours Urea, Urine Urea Nitrogen/24 Hours

January 20, 2025Lab TestsUrine Analysis

Urine 24 hours urea

What sample is needed for urine 24 hours urea?

  1. The test sample is urine.
  2. Collected urine for 24 hours.
  3. The urine sample is stable at 4 to 8 °C for 4 days or preserved with thymol to avoid bacterial action.
    1. Discard the first urine sample and note the time. Add the last sample of urine to the container.
    2. 10 grams of boric acid can be used as a preservative/for 24 hours.

What are the indications for urine 24-hour urea?

  1. This test is mainly used to:
    1. Determine protein metabolism.
    2. It determines the amount of protein needed by severely ill patients.
    3. It gives information about kidney function.
    4. It can monitor kidney disease.
    5. Patients with confirmed or suspected protein digestion and absorption problems.
    6. Patients on long-term enteral nutrition or parenteral nutrition.

How will you discuss the pathophysiology of blood urea?

  1. Urea formula =     
  2. Urea is the major excretory product of protein metabolism.
Urea formation

Urea formation

Urea cycle and role of the liver

Urea cycle and role of the liver

Urea formation

Urea formation

  1. Urea is a low molecular weight substance easily excreted by glomeruli and mostly in the urine.
  2. The kidneys excrete urea, so the excretion of urea can reflect kidney function.
    1. It is synthesized in the liver, carried to the kidney, and filtered through the glomeruli, mostly into the urine.
Urea metabolism and excretion

Urea metabolism and excretion

  1. 40% is reabsorbed by passive diffusion.
  2. The rate of absorption depends upon:
    1. Urine flow rate.
    2. The degree of hydration.
  3. 10 % of urea is excreted through:
    1. GI tract.
    2. Skin.
  4. Blood urea level depends upon:
    1. Renal function and perfusion.
    2. The protein content of the diet.
    3. Amount of protein catabolism.

What is the normal urine urea nitrogen?

Source 1

Urine urea nitrogen

  • 12 to 20 g/day (428.4 to 714 mmol/day).

Another source

  • 6 to 7 G /24 hours ( 60 to 90 mg/dL )
  • Another reference says:
    • 7 to 20 g /total volume
    • 12 to 24 g /24 hours.
  • This also depends upon the protein intake.

What are the causes of decreased urine urea?

  1. Malnutrition.
  2. Too little protein is in the diet.
  3. Kidney dysfunction.
  4. Increased reabsorption.

What are the causes of increased urine urea levels?

  1. Excessive protein intake.

Increased protein catabolism.

Normal urine picture:

Physical features Chemical features Microscopic findings
  1. Color = Pale yellow or amber
  2. Appearance = Clear to slightly hazy
  3. pH = 4.5 to 8.0
  4. Specific gravity = 1.015 to 1.025
  1. Blood = Negative
  2. Glucose = Negative
  3. Ketones= Negative
  4. Protein = Negative
  5. Bilirubin = Negative
  6. Urobilinogen = Negative (±)
  7. Leucocyte esterase = Negative
  8. Nitrite for bacteria = Negative
  1. RBCs = Rare or Negative
  2. WBC = Rare or Negative
  3. Epithelial cells = Few
  4. Cast = Negative (Occasional hyaline)
  5. Crystal = Negative (Depends upon the pH of the urine)
  6. Bacteria = Negative

Questions and answers?

Question 1: How will you define ornithine?
Show answer
Ornithine is a non-proteinogenic amino acid that plays a critical role in the urea cycle, a metabolic pathway responsible for removing excess nitrogen from the body by converting ammonia into urea.
Question 2: How much urea is excreted by the kidneys?
Show answer
>90% is excreted by the kidneys.

Possible References Used
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