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Mycobacterium Tuberculosis:- Part 6 – Pathozyme Tuberculosis test (ELISA)

February 7, 2023Lab TestsMicrobiology

Table of Contents

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  • Pathozyme Tuberculosis test (ELISA)
      • Sample for Pathozyme Tuberculosis test (ELISA)
      • Indication for Pathozyme Tuberculosis test (ELISA)
      • The principle of the Pathozyme Tuberculosis test (ELISA)
      • Procedure for Pathozyme Tuberculosis test (ELISA)
      • Literature references for Pathozyme Tuberculosis test (ELISA)

Pathozyme Tuberculosis test (ELISA)

Sample for Pathozyme Tuberculosis test (ELISA)

  1. This test is done on the serum of the patient.
  2. A random sample can be taken.

Indication for Pathozyme Tuberculosis test (ELISA)

  • This is a rapid diagnostic test for tuberculosis based on immunological reaction.

The principle of the Pathozyme Tuberculosis test (ELISA)

  1. In the Pathozyme Tuberculosis test (ELISA), there are three antibodies, IgM, IgG, and IgA, can be detected.
    1. The PATHOZYME-TB complex test detects serum IgG antibodies to a recombinant 38-kDa antigen specific for  M. Tuberculosis, which is expressed in and purified from E. coli.
    2. The other antigen is the 16 kDa antigen for M. Tuberculosis.
  2. E.coli is the best source to produce this 38-kDa M. Tuberculosis specific antigen.
  3. This test is specific for diagnosing disease due to the M.Tuberculosis complex.

Procedure for Pathozyme Tuberculosis test (ELISA)

  1. To perform the test, the serum is diluted at 1:50.
  2. It detects three types of antigens from three different varieties of Mycobacterium.
    1. The available kit is Pathozyme, Myco IgM, IgG, and IgA.
  3. This is a very specific test for the diagnosis of Tuberculosis.
  4. The sensitivities and specificities of the tests varied widely, with sensitivities ranging from ∼20% to 60% and specificities ranging from ∼65% to 100%.

Literature references for Pathozyme Tuberculosis test (ELISA)

  1. The Patho-TB™ test could be a rapid and inexpensive method for diagnosing pulmonary tuberculosis.
  2. It has given a high sensitivity and negative predictive value.
  3. Concerning the high number of false-positive results, using a confirmatory diagnostic procedure is mandatory.
    1. Some think it can be used as a confirmatory test for tuberculosis.
Pathozyme Tuberculosis test: TB bacilli with AFB stain

Pathozyme Tuberculosis test: TB bacilli with AFB stain


Possible References Used
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