Field Stain A and B

What ate the contents of Field stain?

1. Field stain A
2. Field stain B

• Thin and thick blood films are made and fixed in methanol for one minute.

How do you make Field Stain A (Dark violet color)?

Using Commercially Available Powder.

1. Field stain A commercially available powder = 5 grams
2. Distilled water is heated to 80°C or 60°C for 30 minutes. = 600 mL.

How to make Field stan A from commercial powder?

Field stain A (ready-made powder).

1. Mix the powder into the water until it is dissolved.
2. Filter the solution.
3. Label the date.

What are the reagents of Field Stain A?

1. Methylene blue (analytical grade) = 1.6 grams.
2. Azur = 1 gram.
3. Disodium dihydrogen phosphate anhydrous = 10 grams.
4. Potassium dihydrogen phosphate anhydrous = 12.5 grams.
5. Distilled water = 1000 mL.

What is the procedure for Field stain A preparation?

1. Take Methylene blue (analytical grade) = 1.6 grams.
2. Take Azur = 1 gram.
3. Take Disodium dihydrogen phosphate anhydrous = 10 grams.
4. Take Potassium dihydrogen phosphate anhydrous = 12.5 grams.
5. Distilled water = 1000 mL.
6. Mix both salts (3 and 4) in the water.
7. Add 500 ml from above into a bottle containing glass beads.
8. Add the stain powders (Methylene blue and Azur) and mix well.
9. Then, add the remainder of the solution.
10. Mix well and filter into another clean bottle.
11. Label the bottle Field stain A and also write the date.

How will you prepare Field Stain B (Orange color)?

How will you make Field Stain B from commercially available powder?

1. Field stain B commercially available powder = 4.8 grams.
2. Distilled water is heated to 80°C or kept at 60°C for 30 min. = 600 mL.
3. Mix well the field stain B until it is dissolved.
4. Filter and label the date.

What are the reagents of the Field stain B?

1. Eosin(yellow water soluble) powder = 2.0 grams.
2. Disodium dihydrogen phosphate anhydrous = 10 grams.
3. Potassium dihydrogen phosphate anhydrous = 12.5 grams.
4. Distilled water = 1000 mL.

How will you prepare Field Stain B?

1. Mix both salts (2 and 3) in the distle water (1000 mL) to make the solution.
2. Add 500 ml from above into a bottle containing glass beads.
3. Add the stain powders Eosin and mix well.
4. Then, add the remainder of the solution.
5. Mix well and filter into another clean bottle.
6. Label the bottle Field stain B, and also write the date.

What is the staining Procedure for Field Stains A and B?

1. Fill up two Coplin jars or wide-mouth bottles:
   1. Field Stain A (Blue stain).
   2. Field Stain B (Red stain).
2. Make a blood smear on a clean glass slide, and it is dried in the air.
3. Fix in methanol for one minute or get Spray ‘Easyfix’.
4. Dry in the air.
5. Dip fixed smear to Field Stain B (Red Stain) for 5  to  6 seconds.
6. Wash in running tap water.
7. Dip smear into Field Stain A (Blue Stain) for 10 to 30 seconds (adjust it).
8. Wash in running tap water.
9. Dry at air and see under oil immersion objective.
10. The staining time may be adjusted.

How will you interpret cells in Field stain?

How do you prepare thick and thin smears?

1. Thick and thin blood smears can be made into two separate slides.
2. Put two separate drops of the blood. For a thin smear, put a small drop and a large drop for a thick smear.
3. Spread the drops by the cover glass.

Note: The timings can be adjusted by staining a few slides.

Questions and answers: