HealthFlex
×
  • Home
  • Immunology Book
  • Lab Tests
    • Hematology
    • Fluid analysis
    • CSF
    • Urine Analysis
    • Chemical pathology
    • Blood banking
    • Fungi
    • Immune system
    • Microbiology
    • Parasitology
    • Pathology
    • Tumor marker
    • Virology
    • Cytology
  • Lectures
    • Bacteriology
    • Liver
    • Lymph node
    • Mycology
    • Virology
  • Blog
    • Economics and technical
    • Fitness health
    • Mental health
    • Nutrition
    • Travel
    • Preventive health
    • Nature and photos
    • General topic
  • Medical Dictionary
  • About Us
  • Contact

Chloride (Blood Chloride Cl¯ ) and Cystic Fibrosis

September 1, 2023Chemical pathologyLab Tests

Blood Chloride (Cl–)

Sample for Blood Chloride Cl¯

  1. It is done on the serum or plasma of the patient.
  2. Except for emergency collect fasting samples because there is a slight decrease after the meal.
  3. Chloride is estimated in sweat to rule out cystic fibrosis.
  4. Other samples are CSF and 24 hours of urine.

Purpose of the test (Indications)

  1. As a part of electrolytes, acid-base balance.
    1. It gives status for hydration.
  2. For the diagnosis of cystic fibrosis.

Precautions for Blood Chloride (Cl–)

  1. Separate serum or plasma from the cells, as a change in pH, will alter the distribution of Chloride.
  2. Avoid hemolysis.
  3. Serum, plasma, and urine are stable for one week at 1 to 4 °C or room temperature.
  4. A frozen sample can be kept for one year.
  5. Drugs that may increase the chloride level are ammonium chloride, acetazolamide, cortisones, androgens, and estrogens.
  6. Drugs that may decrease the chloride level are aldosterone, corticosteroids, thiazide diuretics, and loop diuretics.

Definition of blood chloride (Cl–):

  1. Chloride is the most abundant extracellular anion.
  2. The same conditions affect chloride, which affects sodium (the most abundant extracellular cation).

Pathophysiology of  Blood Chloride (Cl–)

  1. Chloride is the most abundant extracellular anion.
  2. Chloride is the major negative electrolyte(anion) in the extracellular fluid.
    1. Chloride with sodium represents the majority of the osmotically active constituents of plasma. Thus, the serum chloride level changes in the same direction as the sodium level, except for a few conditions. If the serum sodium is low, then serum chloride will also be low.
  3. The interstitial plasma fluid of chloride anion is 103 mmol/L.
  4. Its intracellular fluid (RBC) concentration is 45 to 54 mmol/L.
  5. While the intracellular fluid of other tissue is only 1 mmol/L.
  6. Most physicians advise “electrolytes panel or profile,” which includes:
    1. Sodium.
    2. Potassium.
    3. Chloride.
    4. Bicarbonate.
Electrolytes Extracellular fluid (ECF) meq/L Intracellular fluid (ICF)  meq/L
Sodium 142 10
Potassium 5 156
Chloride 104 4
Bicarbonate 24 12

 

Blood Chloride Cl¯: Chloride extracellular and intracellular

Blood Chloride Cl¯: Chloride extracellular and intracellular

Chloride absorption and excretion:

  1. Chloride ions in the food are absorbed entirely in the intestine.
    1. They are filtered from the plasma at the glomerular level and passively reabsorbed, along with Na+ in the proximal tubules.
  2. Chloride interacts with sodium to maintain the osmotic pressure of blood.
  3. Its main purpose is to maintain the electrical neutrality of salt with sodium.
  4. Aldosterone increases the reabsorption of sodium and Chloride to maintain neutrality.
  5. Chloride acts as a buffer to help in acid-base balance.
    1. The concentration of the Cl– changes inversely with the changes in the concentration of HCO3–.
Blood Chloride Cl¯: Chloride functions

Blood Chloride Cl¯: Chloride functions

  1. Chloride is filtered at the glomerulus passively and reabsorbed at proximal tubules. Further absorption at the loop of Henle.
    1. There is a chloride pump in the ascending limb of the loop of Henle.
    2. Sodium is absorbed passively, while Chloride is absorbed actively by the pump.
    3. Excess Chloride is excreted in the urine and sweat.
Chloride excretion and absorption

Chloride excretion and absorption

  1. The chloride concentration in the urine is important in diagnosing metabolic alkalosis.
    1. Metabolic alkalosis can be corrected with saline I/V therapy in case of decreased extracellular water and where the urine concentration of chloride is <15 mmol/L.
    2. Metabolic alkalosis with normal extracellular water will have urine chloride >15 mmol/L and will not respond to saline therapy.

Functions of Blood Chloride (Cl–):

  1. Maintenance of water balance and osmotic pressure with the help of sodium.
  2. Chloride moves into cells in exchange for bicarbonate produced in the cells.
  3. It can maintain electrical neutrality.
  4. It helps as a buffer to help in the acid-base balance.
  5. Anion-cation balance in the extracellular fluid compartment.
  6. Chloride provides electroneutrality, particularly with Na+.
Blood Chloride, (Cl-): Chloride maintains electrolyte balance

Blood Chloride (Cl-): Chloride maintains electrolyte balance.

The normal level of Blood Chloride (Cl–)

Source 1

  1. Serum = 95 to 105 meq / L (98 to 106 mmol/L)
  2. Urine = 110 to 250 meq/ 24 hours
  3. Sweat:
    1. Normal = 5 to 40 meq/L
    2. Marginal value =  30 to 70 meq/L
    3. Cystic fibrosis = 60 to 200 meq/L
  4. CSF :
    1. Infant  = 110 to 130 meq/L
    2. Adult  =  118 to 132 meq/L
      1. These are 15% higher than those in serum.
  5. Saliva without stimulation  =  5 to 20 meq/L

Other Sources 

Sample  meq/L
Serum or plasma
Cord blood 96 to 104
Premature infant 95 to 110
0 to 30 days 98 to 113
Adult 98 to 107
>90 years 98 to 111
Urine 24 hours  meq/24 hours
Infants 2 to 10
<6 years 15 to 40
Male 6 to 10 years 36 to 110
Female 6 to 10 years 18 to 74
Male 10 to 14 years 64 to 176
Female 10 to 14 years 36 to 173
Adult 110 to 250
>60 years 95 to 195
Cerebrospinal fluid meq/L
Infant 110 to 130
Adult 118 to 132
Feces  24 hours  meq/L 
3.2 to ± 0.7
Sweat  meq/L
 Normal 5 to 35
 Marginal 30 to 70
Cystic fibrosis 60 to 200
Saliva meq/L
normal without stimulation 5 to 20

Cystic fibrosis

  1. Cystic fibrosis (mucoviscidosis) or fibrocystic disease of the pancreas is the most common lethal autosomal recessive. an inherited disorder in Europe.
  2. In Europe, it is estimated 1 in 2,000 live births.
  3. In Afro-Americans, the incidence is 2% of that in Europe.
  4. It is rare in Asians.

Pathology of cystic fibrosis:

  1. Around 90% of the homozygotes have symptoms predominantly resulting from damage to mucus-producing glands.
  2. Non-mucus-producing glands may also be affected.
  3. The mucus is very thick, which can plug the ducts and lead to obstructive complications.
  4. In the lungs, it leads to recurrent bronchopneumonia. This is a very serious complication.
  5. Pseudomonas and staphylococcus aureus are the common pathogens.
  6. The next complication is the complete or partial destruction of the exocrine portion of the pancreas, and this will end up in various conditions like:
    1. Malabsorption.
    2. Steatorrhea.
    3. Digestive disturbances.
    4. Malnutrition.
  7. A less common complication is biliary cirrhosis.

Sweat test for cystic fibrosis:

Procedure to collect the sweat:

  1. Sweat is collected in a plastic bag or by iontophoresis.
  2. Sweat is induced by sweat-stimulating agents like the pilocarpine.
  3. Iontophoresis consists of two small electrodes that create a tiny electric current.
  4. Sweat is collected in the small gauze pad.
  5. This procedure is painless.

Sweat test interpretation:

  1. The level of sodium and chloride is higher in patients with cystic fibrosis.
  2. For a definite diagnosis, sweat is collected. The sample should weigh >50 mg, and weighing <50 mg is inadequate.
  3. In children, sweat sodium >70 meq/L and chloride >60 meq/L are abnormal and are diagnostic of cystic fibrosis.
  4. Sodium and chloride may be raised in the first three days of life and then decrease to childhood level by 4th day.
  5. The sample should not be collected from the palm as there is an increased concentration of sodium and chloride (electrolytes).

Signs and symptoms of Hypochloremia:

  1. There is the loss of Cl–, usually resulting from hyponatremia or elevated HCO3– concentration, as in metabolic alkalosis.
    1. This will develop with vomiting and loss of HCL.
    2. Hypochloremia characterizes cystic fibrosis.
    3. Restricted use of salt or in use of diuretics is accompanied by Cl– deficiency.
  2. There is hyperstimulation of the nervous system and muscles.
  3. Shallow breathing.
  4. Hypotension.
  5. Tetany.

Signs and symptoms of Hyperchloremia

  1. This occurs when there is too much sodium or too little bicarbonate.
    1. More than a normal amount of Cl – can be expected with hypernatremia or metabolic acidosis.
    2. Ingestion of excessive Cl– accompanies the use of an ammonium chloride diuretic.
  2. Usually, no specific symptoms are associated with chloride excess.
    1. There are lethargy and weakness.
    2. Deep breathing.

Increased level of Blood Chloride (Cl–) (Hyperchloremia):

  1. urinary tract obstruction, glomerulonephritis, renal tubular acidosis, and acute renal failure.
  2. Diabetes Insipidus.
  3. Salicylate intoxication.
  4. Prolonged diarrhea with the loss of sodium bicarbonate.
  5. Respiratory alkalosis.
  6. Some cases of primary hyperparathyroidism.
  7. Maybe because of excessive intake.
  8. Eclampsia.
  9. Cushing syndrome.
  10. Renal tubular acidosis.
  11. Dehydration.
  12. Due to the excessive infusion of normal saline.
  13. Hyperventilation.

Decreased level of Blood Chloride (Cl–) (Hypochloremia):

  1. excessive sweating.
  2. Prolonged vomiting.
  3. Gastric suction.
  4. Salt losing nephritis.
  5. Addisonian crises.
  6. Metabolic acidosis is associated with increased organic anions.
  7. Aldosteronism.
  8. Respiratory acidosis.
  9. Water intoxication.
  10. Diuretic therapy.
  11. Hypokalemia.
  12. Burn
  13. Overhydration.

Serum electrolytes in various conditions:

Clinical condition pH Chloride meq/L Sodium meq/L Potassium meq/L Bicarbonate meq/L
Normal 7.35 to 7.45 100 to 106 136 to 145 3.5 to 5.0 24 to 26
Diabetic acidosis 7.2 80 122 5.6 10
Severe diarrhea 7.2 96 128 3.2 12
Vomiting 7.6 94 150 3.2 38
Respiratory acidosis 7.1 80 142 5.5 30
Respiratory alkalosis 7.6 112 136 5.5 14

Acidosis and alkalosis:

Clinical conditions pH Chloride meq/L Bicarbonate meq/L Sodium meq/L pCO2 (mm Hg)
Normal 7.40 105 25 14040
Metabolic acidosis 7.30 115 15 140 31
Metabolic alkalosis 7.49 92 36 140 48
Respiratory alkalosis (Chronic) 7.44 102 25 136 40
Respiratory acidosis (Chronic) 7.37 100- 102 28 140 50
  1. Mixed metabolic acidosis
  2. Mixed chronic respiratory alkalosis
7.39 108 14 136 24
  1. Mixed metabolic alkalosis
  2. Chronic respiratory acidosiss
7.4 90 40 140 67
  1. Mixed metabolic acidosis
  2. Metabolic alkalosis
7.4 103 25 140 40

Critical values of chloride in serum or plasma are:

  • High value = >115 meq/L.
  • Low value = <80 meq/L.

Questions and answers:

Question 1: What is iontophoresis?
Show answer
This is the procedure by which two small electrodes are applied to collect the sweat.
Question 2: How much is the chloride in the cells (intracellular)?
Show answer
Intracellular concentration of the chloride is 4 meq/L.

Possible References Used
Go Back to Chemical pathology

Add Comment Cancel



The reCAPTCHA verification period has expired. Please reload the page.

  • Lab Tests
    • Blood banking
    • Chemical pathology
    • CSF
    • Cytology
    • Fluid analysis
    • Fungi
    • Hematology
    • Immune system
    • Microbiology
    • Parasitology
    • Pathology
    • Tumor marker
    • Urine Analysis
    • Virology

About Us

Labpedia.net is non-profit health information resource. All informations are useful for doctors, lab technicians, nurses, and paramedical staff. All the tests include details about the sampling, normal values, precautions, pathophysiology, and interpretation.

[email protected]

Quick Links

  • Blog
  • About Us
  • Contact
  • Disclaimer

Our Team

Professor Dr. Riaz Ahmad Bhutta

Dr. Naheed Afroz Syed

Dr. Asad Ahmad, M.D.

Dr. Shehpar Khan, M.D.

Copyright © 2014 - 2025. All Rights Reserved.
Web development by Farhan Ahmad.