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Anemia:- Part 9 – Sideroblastic Anemia, and Anemia Due To Chronic Diseases

July 2, 2023HematologyLab Tests

Table of Contents

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  • Sideroblastic anemia:
      • Sample for Sideroblastic Anemia
      • Definition of sideroblastic anemia:
      • Pathophysiology of sideroblastic anemia:
      • Classification of sideroblastic anemia:
      • Signs and symptoms of sideroblastic anemia:
      • Lab findings of sideroblastic anemia are:
      • Treatment of sideroblastic anemia:
  • Anemia due to chronic diseases
      • Definition of anemia due to chronic diseases:
        • Table showing findings in various anemias:
        • Characteristic Findings in Various Anemias:
        • Anemia type
          • HB
          • MCV 
          • MCHC
          • Ferritin 
          • Iron binding capacity
          • serum iron
        • Classification of anemia based on RDW:
        • Abnormalities of RBCs morphology and their etiology:
        • Questions and answers:

Sideroblastic anemia:

Sample for Sideroblastic Anemia

  1. EDTA blood is needed.
  2. Bone marrow smear for iron stain.

Definition of sideroblastic anemia:

  1. Sideroblastic anemia indicates a group of disorders with the presentation of:
    1. Anemia.
    2. Ineffective erythropoiesis.
    3. Increased serum and tissue iron.
  2. Increased number of ringed sideroblastic RBCs in the bone marrow aspirate (at least 20%).
  3. Sideroblasts are normoblasts with abnormal stainable iron in the cytoplasm that form a ring around the nucleus.
    1. Normoblast contains ferritin. These cells are absent in iron deficiency anemia and anemia due to chronic infection.
  4. Siderocytes are normal reticulocytes that contain iron in the form of ferritin or hemosiderin in the mitochondria. These cells have no diagnostic importance.
Sideroblast in the bone marrow

Sideroblast in the bone marrow

Sideroblastic cells

Sideroblastic cells

  1. These anemias are due to abnormalities of heme synthesis.
  2. To qualify the sideroblastic anemia, >15% of ringed sideroblasts are to be found in the following:
  3. Congenital (inherited):
    1. This includes sex-linked congenital sideroblastic anemia.
    2. Autosommal recessive sideroblastic anemia.
  4. Acquired (drug-induced):
    1. Primary or idiopathic.
      1. Myelodysplasia.
    2. Secondary.
      1. Lead.
      2. Alcoholism.
      3. Thalassemia.
      4. Drugs include isoniazid and chloramphenicol.
      5. This may be seen in malignancies or malabsorption.

Pathophysiology of sideroblastic anemia:

  1. The pathogenesis is incompletely understood.
  2. It is due to abnormalities of heme metabolism.
  3. Several investigators found enzyme deficiency, including the deficiency of ALA synthase and uroporphyrinogen decarboxylase in these patients.
  4. Irrespective of the etiology, there is an abnormal deposition of iron or siderotic granules in normoblast mitochondria. The mitochondria are present around the nucleus.
  5. The body has an adequate amount of iron, but it cannot incorporate into hemoglobin.
  6. The iron (Fe) enters the developing RBCs but accumulates in the normoblast’s perinuclear mitochondria in primary sideroblastic anemia.
sideroblast and siderocyte in anemia

sideroblast and siderocyte in anemia

Classification of sideroblastic anemia:

A. Inherited type:

  1. This is rare and is sex-linked.
  2. Sex-linked (X-linked) anemia is more common than Autosomal recessive sideroblastic anemia.
  3. The responsible gene is ALAS2, found on the sex chromosome X.
    1. Pyridoxine-responsive.
    2. Pyridoxine-refractory.
    3. Autosomal, pyridoxine-refractory.
  4. These sideroblastic anemias are usually present in childhood.
  5. MCV is usually low, and RDW is usually high.

B. Acquired type:

  1. Primary (idiopathic).
    1. Myelodysplasia.
  2. Secondary:
    1. Associated with myeloproliferative disorders like leukemia and polycythemia vera.
    2. Pyridoxine deficiency (responsive anemia):
      1. Alcoholism.
      2. Drugs induced like INH, cycloserine, chloramphenicol, and chemotherapy.
      3. Vitamin B12 deficiency.
    3. Hemoglobin synthesis defects:
      1. Deficiency of vitamin B12 and folic acid.
      2. Erythropoietic porphyria.
      3. Lead poisoning.
    4. Radiation.
    5. Other diseases are e.g., rheumatoid arthritis, carcinoma, megaloblastic,  hemolytic anemia, hemolytic anemia, and malabsorption.
    6. A specially acquired rare form of Pearson syndrome has sideroblastic anemia, pancreatic insufficiency, and copper deficiency.
  3. Pyridoxine-responsive anemia:
    1. Classical type.
    2. Variant forms.

Signs and symptoms of sideroblastic anemia:

  1. Typically anemia appears early, usually within the first few months or years of life.
  2. The patients will show pallor and splenomegaly in the sideroblastic anemia.

Lab findings of sideroblastic anemia are:

  1. The diagnostic feature is nucleated RBCs with iron granules called ringed sideroblasts, present in the bone marrow and dimorphic picture in the peripheral blood smear.
  2. Hemoglobin is low.
    1. MCV is variable and may be low, normal, or increased.
    2. MCH and MCHC are often low but may be normal.
    3. White blood cells may show abnormality.
    4. RDW is increased.
  3. Serum iron is usually more than normal. There may be increased or normal iron stores.
  4. Ferritin level is also increased.
  5. Transferrin% saturation is high.
  6. The serum ferritin level is markedly raised.
  7. The serum B12 and folic acid levels are normal.
  8. Raised bilirubin level.
  9. LDH is raised.
  10. Decreased serum haptoglobin.
  11. Peripheral blood smears:
    1. It shows a dimorphic picture with the presence of normocytic (normal RBCs) and microcytic and hypochromic RBCs (small-size RBCs). Occasional macrocytes are seen.
    2. Microcytosis is more common in the inherited form of sideroblastic anemia.
    3. Peripheral blood smears show hypochromic anemia, which is microcytic, normochromic, or macrocytic (dimorphic picture).
    4. The dimorphic picture is seen in the primary type. It is prominent anisopoikilocytosis.
    5. The microcytic form is seen mostly in the inherited form of sideroblastic anemia. In comparison, the macrocytic form is seen in the acquired form.
    6. There may be seen iron-containing Papenheimer bodies, and may look like basophilic stippling.
Sideroblastic anemia

Sideroblastic anemia

  1. Bone marrow:
  2. It shows erythroid hyperplasia, but circulating reticulocytes are not increased (ineffective erythropoiesis).
  3. There are sideroblasts in the bone marrow aspirate. and these are >15%.
  4. Cytogenetics study shows a chromosomal anomaly in 25% to 50% of the cases.
  5. There are increased or normal iron stores.

Treatment of sideroblastic anemia:

  1. Treat the cause. Some patients respond when alcohol and drugs are stopped.
  2. In the primary type, these are unresponsive to various vitamins, especially pyridoxine (another reference says some patient responds to pyridoxine).
  3. Folic acid may be given in case of folate deficiency.

Anemia due to chronic diseases

Definition of anemia due to chronic diseases:

  1. This anemia occurs mostly in chronic inflammatory and malignant diseases.
  2. The anemia is present for several months following chronic diseases (which is present for several months).
  3. These are commonly associated with infections, malignant neoplasms, and autoimmune disorders.
  4. It is usually normocytic and either normocytic or hypochromic anemia.

Pathogenesis of anemia due to chronic diseases:

  1. The mechanism of this anemia is not clearly understood.
  2. The basic defect is in the iron utilization for erythropoiesis.
  3. It looks like there is a block of iron delivery from the reticuloendothelial system to RBCs.
  4. A chronic disease state blocks the transfer of stored iron to maturing erythroid precursors within the bone marrow.
  5. This will lead to iron deficiency in RBCs while the stores have abundant iron.
Anemia of chronic diseases

Anemia of chronic diseases

  1. There is a decreased release of iron from the macrophages to plasma.
  2. RBCs’ life span is reduced.
  3. Inadequate erythropoietin response to anemia is caused by the effect of cytokines like IL-1 and tumor necrosis factor (TNF) on erythropoiesis.
  4. Hepcidin, released by the liver in response to inflammation, will inhibit macrophages’ iron release and absorption.
  5. The anemia will respond to successful treatment of the cause but no response to iron therapy.
  6. Anemia due to chronic diseases may be due to:
    1. Decreased Erythropoietin response by the RBCs.
    2. Decreased RBCs survival.
    3. Defective iron absorption.
    4. Cytokines block the release of iron from the reticuloendothelial system for the development of RBCs.

Causes of anemia due to chronic diseases:

  1. This is seen in collagen diseases (autoimmune diseases).
    1. Systemic lupus erythematosus.
    2. Rheumatoid arthritis.
    3. Sarcoidosis.
  2. Inflammatory chronic conditions.
    1. Tuberculosis.
    2. Chronic osteomyelitis.
    3. Fungal infection.
  3. Malignancies.
    1. Carcinoma.
    2. Lymphomas.
    3. Multiple myeloma.

Causes of anemia due to chronic diseases:

Group of the anemia Causes of the chronic diseases
  • Infections
  1. Tuberculosis
  2. Chronic osteomyelitis
  3. Fungal infections
  4. Bronchiectasis
  5. Lung abscess
  6. Empyema
  7. Infective endocarditis
  8. Brucellosis
  • Autoimmune diseases
  1. Systemic lupus erythematosus (SLE)
  2. Sarcoidosis
  3. Rheumatoid arthritis
  4. Inflammatory bowel disease
  5. Vasculitis
  • Malignancies
  1. Lymphomas
  2. Multiple myeloma
  3. Carcinomas
  • Other causes
  1. Chronic liver disease
  2. Chronic adrenal insufficiency
  3. Hypothyroidism

Signs and symptoms of anemia due to chronic diseases:

  1. Anemia appears for several months after the chronic disease.
  2. Anemia usually presents 1 to 3 months following the onset of chronic disease.

Lab findings of anemia due to chronic diseases:

  1. There is Low hemoglobin (7 to 11 g/dL).
  2. MCV is normal.
  3. RBCs may be normal or microcytic and hypochromic RBCs. This microcytosis is not as severe as iron deficiency anemia.
  4. Usually normocytic RBCs with normal MCV, rarely  MCV is <75 fl.
  5. In some cases, we may see hypochromic or normochromic RBCs.
Anemia of chronic diseases

Anemia of chronic diseases

  1. Decreased Serum iron.
  2. TIBC is normal or decreased.
  3. The serum ferritin is normal or increased.
  4. There is a decreased % saturation.
  5. Normal to increased serum ferritin level.
  6. It needs to differentiate from iron deficiency anemia.
  7. Decreased sideroblastic cells (rare to absent ringed sideroblast).

Lab findings of the anemia due to chronic diseases:

Lab test Clinical parameters
  • RBC size
  • Normocytic
  • MCH
  • Normal
  • Reticulocyte count
  • Low/normal
  • Serum iron
  • Variable
  • TIBC
  • Normal
  • Ferritin level
  • Normal
  • Folic acid level
  • Normal
  • B12
  • Normal

Table showing findings in various anemias:

Type of anemia Hb MCV MCH MCHC
Iron deficiency low low low low
Megaloblastic low high high normal
Thalassemia low low low low
Chronic illness low low low low

Characteristic Findings in Various Anemias:

Anemia type

HB
MCV 
MCH
MCHC
Ferritin 
Iron binding capacity
serum iron
 RDW
Iron deficiency low low <76 fl low low/normal decreased increased decreased increased
Megaloblastic low high >100 fl/cell

increased

>32 pg

low 32 to 36 g/dL  raised/normal increased
Chronic illness low low/normal low low normal/ increased normal / decreased decreased normal
Alpha Thalassemia low or normal low low low  normal /increased  normal

 normal or

increased

increased
Beta Thalassemia low low low low  increased/normal  normal  increased/normal  increased
Aplastic anemia low increased normal  normal normal

Classification of anemia based on RDW:

 Cell size Normal  RDW High RDW
   Microcytosis
  1. Thalassemia minor
  2. Chronic diseases,
  3. Some hemoglobinopathy trait
  1. Iron deficiency
  2. Hb H
  3. Anemia of chronic diseases
  4. some cases of thalassemia.
   Normocytic
  1.  Hereditary spherocytosis
  2. Acute bleeding
  3. some chronic diseases
  4. Some Hb traits
  1. Some early or partially treated iron deficiency anemia
  2. Sickle cell anemia
   Macrocytosis
  1. Aplastic Anemia
  1. Autoimmune hemolytic anemia
  2. vit. B or folate deficiency
  3. Liver disease
  4. thyroid disease
  5. Myelodysplasia
  6. Alcohol use

Abnormalities of RBCs morphology and their etiology:

Type of RBCs abnormality Etiology for the abnormality

Microcytic

Anemia Microcytic hypochromic

Sickle cell Hb structure

  1. Iron-deficiency anemia
  2. Thalassemia
  3. Sideroblastic anemia
  4. Lead poisoning
MacrocyticAnemia macrocytic megaloblastic
  1. Megaloblastic anemia
  2. Liver diseases
  3. Myelodysplastic syndrome
  4. Increased reticulocyte count
SpherocytesRBC spherocyte
  1. Hereditary spherocytosis
  2. Hemolytic anemia
  3. Post transfusion

Target cells

RBC target cell

RBC target cell

  1. Thalassemia
  2. Liver diseases
  3. Sideroblastic anemia
  4. Hemoglobinopathies
Teardrop cells   RBC tear drop poikilocyte
  1. Severe anemia
  2. Pernicious anemia
  3. Myeloproliferative anemia
Elliptocytes     RBC elliptocyte
  1. Hereditary elliptocytosis
  2. Thalassemia
  3. Iron-deficiency anemia
Sickle cellsSickle cell RBCs
  1. Sickle cell anemia
  2. Sickle-thalassemia
Stomatocytes  RBC Elliptocyte
  1. Malignant tumors
  2. Acute alcoholism
Burr cells         RBC burr cells
  1. Renal diseases
  2. Liver diseases
  3. Bleeding gastric ulcer
  4. Severe burns
Acanthocytes  RBC acanthocyte
  1. Alcohol intoxication
  2. Post splenectomy
  3. Vitamin E deficiency
  4. Congenital abetalipoproteinemia
Hemet cells    RBC Helmet cell
  1. G-6-PD deficiency
  2. Pulmonary emboli
Schistocytes   RBC Schistocyte cell
  1. Hemolytic uremic syndrome
  2. Thrombotic idiopathic thrombocytopenia (ITP)
  3. Disseminated intravascular coagulopathy (DIC)

Summary of lab findings in various anemias:

Lab test Iron-deficiency anemia Pernicious anemia Folic acid deficiency Aplastic anemia Thalassemia Sideroblastic anemia Hemolytic anemia Post hemorrhagic anemia Anemia of chronic diseases
Hemoglobin Low Low Low Low or normal Low Low Low Normal or low Low
Hematocrit Low Low Low Low or normal Low Low Low Normal or low Low
MCV Low High High A normal or mild increase Low Low Normal or high Slightly low Low or normal
Reticulocytes count A normal or mild increase Low Low Low Increased A normal or mild increase High Increased Normal
Plasma Iron Low Increased Increased Increased Increased or normal Increased Normal or high Normal Low
TIBC Increased Normal Normal Normal Normal Normal Normal Normal Low
Ferritin level Low Increased Increased Normal Increased or normal Increased Normal Normal Normal
Folate level Normal Normal Low Normal Normal Normal Normal Normal Normal
Serum B12 level Normal Low Normal Normal Normal Normal Normal Normal Normal
Transferrin Low Mild increase Mild increase Normal Increased Normal Normal Mildly low
Bilirubin Increased

Questions and answers:

Question 1: What is peripheral blood picture of sideroblastic anemia.
Show answer
It shows a dimorphic picture of RBCs.
Question 2: What is the mechanism of anemia due to chronic diseases?.
Show answer
Its mechanism is not clearly understood. The basic defect is in the iron utilization for erythropoiesis.
Possible References Used
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