α-Hydroxybutyrate Dehydrogenase (HBDH)

Sample α-Hydroxybutyrate Dehydrogenase

1. This is done on the serum of the patient.
2. Separate serum as soon as possible.
3. Avoid hemolysis and use a clear serum.
4. Plasma Is not recommended.

Purpose of the test (Indications) for α-Hydroxybutyrate Dehydrogenase

• For the diagnosis of myocardial infarction.

Pathophysiology of α-Hydroxybutyrate Dehydrogenase

1. The HBDH is an alternate to determine the  LDH isoenzymes (LDH-1).
2. HBDH indirectly measures LDH-1 of heart activity.
3. HBDH is cheaper than the LDH isoenzyme assay.
4. Roasalki and Wilkinson advocated the ratio of LDH/HBDH.
   1. In healthy individual serum, the ratio of LDH/HBDH varies from 1.2 to 1.6.
   2. in liver parenchymal diseases is 1.6 to 2.5.
   3. While in heart disease (Myocardial infarction), when LDH-1 and LDH-2 activity is increased and at the same time HBDH is also increased, then the LDH/HBDH ratio is decreased to 0.8 to 1.2.
   4. LDH/HBDH ratio is more popular in the UK than in the USA.
5. The level of HBDH is usually detected within 12 hours of the infarction.
6. The peak level is seen from 48 to 72 hours.

Normal of α-Hydroxybutyrate Dehydrogenase

• At 25 C = 68  to 135 U/L.
• At 30 C = 82 to 163 U/L.
• At 37 C = 96 to190 U/L.

The increased level is seen in the following:

1. Myocardial infarction. HBDH can be done to assess the damage to the heart muscles.
2. It is raised in liver cell damage.
   1. It is raised in hepatitis.
   2. It is raised in cirrhosis.
   3. It is raised in drug-induced liver cell injury.
3. It is also raised in kidney diseases.
4. Muscular dystrophy.
5. Megaloblastic anemia.
6. Disseminated metastasis. It can be a tumor marker for lung, liver, and colorectal carcinoma.

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